The chromatogram obtained with solution (1) shows in the lower part a dark blue band due to menthol

. The chromatogram obtained with solution (2) shows a large blue band due to triacontanol (melissyl alcohol) at an Rf value between those of the bands due To menthol and thymol in the chromatogram obtained with solution (1) and blue bands at Rf values between those of the bands due to menthyl acetate and thymol in the chromatogram obtainsd with solution (1) In addition, the chromatogram obtained with solution (2) shows further bands at higher Rf values than menthyl acetate, that wrth the highest Rf value being very pronouncedH and a number of faint bands below that due to tnaconlanol; a band on the line of application is blue. Melting range; Between 78° and 88°, determined by Method II. Appendix 8. 8. Heavy metals: Not more than 40 ppm, determined on 0.5 g by Method B. Appendix 3. 12. Acid value Not more than 12.0, determined by the following method Weigh accurately about 2 g (w) in a flask fitted with a reflux condenser, add 40 ml of xylene and heat until the substance has dissolved. Add 20 ml of ethanol (95%) and titrate the hot solution with 0. 5M ethanolic potassium hydroxide, using phenolphthaleini solution as indicator, until a pink colour persists for at least 10 seconds (n ml). Repeat be operation without the substance being examined (n2 ml). Calculate the acid value from the expression 28Q5(n1n2)/w. Saponification value: Between 78 and 95. determined by the following method. To the titrated solution from the determination of the Acid value, add 20. 0 ml of .0.5M ethanolic potassium hydroxide and boil under a reflux condenser for 3 hours. Titrate the hot solution immediately with 0.5M hydrochloric acid, using 1 ml. of phenolphthalen solution as indicator, until the red colour is discharged. Reheat the solution to boiling and continue the titration, if necessary, until the red colour no longer reappears on healing (n3 ml). Repeat the operation without the substance being examined (n4 ml). Calculate the saponification value from the expression a+[28. 05(n4-n3)/w where a is the acid value. Sulphaled ash: Not more than 0. 25%. determined on 2 g, Appendix 3. 22.CASTOR OlLCastor Oil is the fixed oil obtained by cold expression from the seeds of Ricinus communis Linn (Fam. Euphorbiaceae). It may contain suitable antioxidants. Category Laxative; pharmaceutical aid (plasticiser). Dose: 5 to 15 ml.Description: Pale yellowish or almost colourless, transparent, viscid liquid, odour, slight and characteristic Solubility:Miscible with chloroform, with ethanol (95%). with ether and with qlactal acetic acid, slightly soluble in light petroleum. Storage: Store m well-filled, well-closed.